Combination of Thin Layer Chromatography (TLC) With other Techniques

 COMBINATION OF TLC WITH OTHER TECHNIQUES 

TLC may be combined with column chromatography and vapour-phase chromatography as discussed here briefly :

 

(a) Column Chromatography : 

TLC helps in selecting the best combination of solvent and adsorbent for a given column separation. Miller and Kirchner**** (1952) developed this combination thoroughly and employed it extensively for the separation of a large number of difficult types of compounds.

 

(b) Vapour-phase Chromatography : 

The various spots obtained from TLC may be eluted, concentrated and then subjected to vapour-phase chromatogrphy studies. Ikeda et al***** (1961, 1962) exploited this combination for the analysis of a variety of naturally occurring constituents, namely :

 

(i) Citrus oils and other essential oils,

 

(ii) Oestrogens in urine sample,

 

(iii) Testosterone in urine sample, and

 

(iv) Progesterone in plasma.